核心儀器設施中心將於5月27日10:00~12:00於B1-R1-1122演講廳舉辦『Nikon超解析影像暨全內反射螢光顯微鏡系統說明會(N-SIM)』教育訓練。此系統超越了光學影像極限，將XYZ解析力提升兩倍，可截取115nm微小蛋白質影像(可解析粒線體大小的結構變化等)，進而清楚辨別蛋白間相對位置之改變。另外此系統具有TIRF-SIM顯微影像技術，相較於傳統的TIRFM(Total Internal Reflection Fluorescence Microscope)提供了更高光學解像力的全內反射螢光觀察，可獲得細胞膜附近更多的細節訊息。目前系統多架設了2顆長工作距離鏡頭(10倍及20倍)，方便進行長時間活細胞觀察。此Nikon超解析影像暨全內反射螢光顯微鏡系統僅開放給認證過的使用者自行上機。欲自行操作的使用者請務必參加此次訓練課程，並於報名同時標明參加上機課程(預定每組上機時間為50~60分鐘，每組3人。上機課程時間將依報名人數調整。若有特殊上機時段需求，請於報名時加註，謝謝!)。未來自行上機使用者須通過第二階段認證才可單獨上機操作。第一階段：上過教育訓練課程或看過教學影片。第二階段：通過實驗室管理者或廠商認證。

應用領域 :

Neuroscience、Immunology(Regenerative medicines)、Developmental biology(iPS cell)、Cell biological study(Gene manipulation)、Physiology(Molecularly-targeted drug)

 日期：109年5月27、28日（週三、四）

地點：研究大樓B1 R1-1122演講廳（5/27上課）

      研究大樓 R2-5041顯微鏡室（5/27、5/28分組上機）

時間：5/27 10：00 ~ 12：00 N-SIM上課課程

        N-SIM系統硬體介紹
        SIM基本原理介紹
        SIM-2D/3D的原理及樣品製作要件
        SIM-TIRF 的原理及適用情況
        螢光影像拍攝
        電動化多通道螢光拼圖
        多點多通道活細胞長時間觀測
        基礎影像處理功能介紹

      5/27  14：00-16：00分組上機研習（每組60分鐘）

      5/28  10：00-12：00分組上機研習（每組60分鐘）

            14：00-16：00分組上機研習（每組60分鐘）

 報名截止日：5/26日中午12時

報名方式：e-mail：shufenhu@nhri.org.tw（胡淑芬）【請於信件內註明姓名、單位組別、分機、是否參加上機課程】

若有任何疑問，請聯絡胡淑芬  分機33702、33703

    NHRI Optical Biology Core Facility is scheduled to host a training session for the Nikon N-SIM Super Resolution Microscope System on May 27 and 28. This system nearly doubles the spatial resolution of conventional light microscopes to approximately 115 nm, and enables detailed visualization of minute intracellular structures and their interactions. In addition, this system has TIRF-SIM microscopic imaging technology. Compared with the traditional TIRFM (Total Internal Reflection Fluorescence Microscope), it provides more internal reflection fluorescence observation with higher optical resolution, and more detailed information near the cell membrane can be obtained. At present, the system has set up two long working distance lenses (10 times and 20 times) to facilitate long-term living cell observation.

This Nikon Super Analytical Imagery and Total Internal Reflection Fluorescence Microscope System is only open to certified users on their own. Users who want to operate by themselves must participate in this training course, and sign up to attend the on-site demonstration section (3 people in each group, the scheduled time for each group is about 50~60 minutes but it can be adjusted according to the number of applicants). If you have a special demand for the on -site section, please raise it when you register. Users who are planning to use N-SIM microscope independently have to pass two stages of certification before they can operate on their own. The first stage is to attend an education training course or watched a teaching film. The second stage is to be certified on site by the laboratory manager or manufacturer.

 Area of applications: Neuroscience、Immunology(Regenerative medicines)、Developmental biology(iPS cell)、Cell biological study(Gene manipulation)、Physiology(Molecularly-targeted drug)

 The detailed course content is as follows.

 Time：Lecture(in Chinese)：5/27 10:00~12:00

  Demonstration：5/27 14:00~16:00

                             5/28 10:00~16:00

Venue：Lecture ：R1-B1122

              Demonstration：R2-5041

 Time：5/27 10:00 ~ 12:00 N-SIM lecture

            N-SIM system hardware introduction

             Introduction to SIM basic principles

             Principle of SIM-2D/3D and sample preparation requirements

             Principle and application of SIM-TIRF

             Fluorescent image capture

             Motorized multi-channel fluorescent puzzle

             Long-term observation of multi-point multi-channel living cells

             Basic image processing function introduction

    5/27 14:00-14:55 demonstration session (1)

                 15:00-15:55 demonstration session (2)

     5/28 10:00-10:55 demonstration session (3)

                 11:00-11:55 demonstration session (4)

                 14:00-14:55 demonstration session (5)

                 15:00-15:55 demonstration session (6)

 Registration deadline: May 26 noon

Registration E-mail: shufenhu@nhri.org.tw (Please write down your name, tel. Ext, and division)

 If you have questions regarding this training session please call us:

Hu Shu-Fen, Tel: ext. 33702, 33703

 新冠肺炎防疫期間，敬請參加教育訓練同仁遵守以下原則:

1.      進入會場前所有人員應先自行量測體溫、並作手部消毒清潔，若發燒(耳溫≧38℃、額溫≧37.5℃)則不可進入會場。

2.為降低風險，入場時請同仁配戴口罩，並以間隔或分散方式就座。